ABC2036

Anti-MLKL

from rabbit

• Synonym(s): Mixed lineage kinase domain-like protein, hMLKL
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_689862
• UniProt accession no.: Q8NB16
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: human ... MLKL(197259)

Description

General description

Mixed lineage kinase domain-like protein (UniProt: Q8NB16; also known as hMLKL) is encoded by the MLKL gene (Gene ID: 197259) in human. MLKL is a pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. MLKL contains a protein kinase-like domain; but it lacks several residues required for activity. Its ATP-binding site is located within amino acids 209-217. Two isoforms of MLKL have been described that are produced by alternative splicing. It contains two coiled coil regions (aa 55-84 and 139-180) and coiled coil region 2 is shown to be responsible for its homotrimerization. MLKL is phosphorylated on Thr357/Ser358 by RIP3 kinase that leads to its oligomerization. The oligomeric MLKL then translocates from cytosol to plasma membrane and intracellular membranes where it bind to phosphatidylinositol and cardiolipin and disrupts membrane integrity leading to necrotic cell death. MLKL is reported to play a critical role in TNF-induced necroptosis. (Ref.: Wang, H., et al (2014). Mol. Cell 54(1); 133-146).

~52 kDa observed; 54.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

Epitope: N-terminus

GST/His-tagged recombinant fragment corresponding to the first 124 amino acids from the N-terminal region of human Mixed lineage kinase domain-like protein (MLKL).

Application

Anti-MLKL, Cat. No. ABC2036, is a rabbit polyclonal antibody that detects Mixed lineage kinase domain-like protein (MLKL) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Apoptosis & Cancer

Western Blotting Analysis: A 1:500 dilution of from a representative lot detected MLKL in 10 µg of Jurkat cell lysate.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MLKL in human tonsil and human squamous cell carcinoma tissue sections.

Biochem/physiol Actions

This rabbit polyclonal antibody detects Mixed lineage kinase domain-like protein in human cells. It targets an epitope within the first 124 amino acids from the N-terminal region.

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected MLKL in 10 µg of HT-29 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1