AB4336

Anti-SCA-1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, T-cell-activating protein, Stem cell antigen 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 10

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, mouse
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_034868
• UniProt accession no.: P05533
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Ly6A(110454)

Description

General description

Stem cell antigen 1 (SCA-1) is a membrane protein belonging to the Ly-6 family of proteins and contains a single UPAR/Ly6 domain. SCA-1 is involved in the activation of T-cells and is thought to have a critical role in the differentiation, proliferation, and survival of a variety of stem cells. Recent studies have indicated SCA-1 to be an ideal marker for the isolation and purification of stem cells for research and further characterization. It is expressed by both progenitor and stem cells from several tissue types including; cardiac, hematopoietic, mammary, muscle, skin and testis.

~14 kDa observed

Immunogen

Epitope: UPAR/Ly6 domain

KLH-conjugated linear peptide corresponding to mouse SCA-1 at the UPAR/Ly6 domain.

Application

Anti-SCA-1 Antibody is an antibody against SCA-1 for use in WB & IC.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected SCA-1 in rat mesenchymal stem cells.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

Biochem/physiol Actions

No Homology to human was found.

This antibody recognizes SCA-1 at the UPAR/Ly6 domain.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse lung tissue lysate

Evaluated by Western Blot in mouse lung tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected SCA-1 in 10 µg of mouse lung tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable