AB3517

Anti-Polyhistidine Tag Antibody

Chemicon^®, from chicken

• UNSPSC Code: 12352203
• NACRES: NA.43
• eCl@ss: 32160702
• Clone: polyclonal
• Technique(s): ELISA: suitable, immunocytochemistry: suitable, western blot: suitable
• Application: ELISA, ICC, WB
• Citations: 1

Properties

• biological source: chicken
• Quality Segment: 100
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity (predicted by homology): all
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunocytochemistry: suitable, western blot: suitable
• shipped in: dry ice
• target post-translational modification: unmodified

Description

Immunogen

Synthetic peptide consisting of six histidines.

Application

Anti-Polyhistidine Tag Antibody detects level of Polyhistidine Tag & has been published & validated for use in ELISA, WB & IC.

Immunoblotting: 1:250-1:2,000

Immunocytochemistry: 1:250-1:1,000

ELISA: 1:250-1:2,000

Optimal working dilutions must be determined by the end user.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

Biochem/physiol Actions

Polyhistidine tag, 6-His Tag containing proteins

Physical form

Affinity purified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable