8.56044

Fmoc-Cys(Trt)-NovaSyn^® TGT

for peptide synthesis, Novabiochem^®

• Synonym(s): Trityl-Protected Cysteine Resin
• UNSPSC Code: 12352209
• NACRES: NA.22

Properties

• Product Name: Fmoc-Cys(Trt)-NovaSyn^® TGT, Novabiochem^®
• Quality Segment: 200
• product line: NovaSyn^® TG, Novabiochem^®
• form: solid
• reaction suitability: reaction type: Fmoc solid-phase peptide synthesis
• manufacturer/tradename: Novabiochem^®
• application(s): peptide synthesis
• functional group: Fmoc
• storage temp.: 15-25°C

Description

General description

Pre-loaded resin for synthesis of peptide acids and protected peptide fragments containing a C-terminal cysteine amino-acid residue by Fmoc SPPS. The base NovaSyn^® TG is a composite of low cross-linked polystyrene and 3000-4000 M.W. polyethylene glycol. Peptide synthesis is carried out at the ends of the PEG chains that have been functionalized with the hyper-acid labile 4-carboxytrityl alcohol linker. This use of this linker helps prevent racemization and β-piperidinylalanine formation during chain extension.Treatment of the peptidyl resin with 20% TFE in DCM or 1% TFA in DCM cleaves the product from the resin without affecting the standard TFA-labile side-chain protecting groups. Standard TFA cleavage releases the fully deprotected peptide.

Associated Protocols and Technical Articles:
Cleavage and Deprotection Protocols for Fmoc SPPS

Literature references:
^[1] E. Atherton, et al. in "Peptides 1990, Proc. 21st European Peptide Symposium, E. Giralt & D. Andreu(Eds), 1991, Escom, Leiden, pp. 243
^[2] J. Lukszo, et al. (1996) Lett. Pept. Sci., 3, 157.
^[3] Y. Fujiwara, et al. (1994) Chem. Pharm. Bull., 42, 724.
^[4] K. Barlos & D. Gatos in “Fmoc solid phase peptide synthesis: a practical approach”, W. C. Chan & P. D.White (Eds.), Oxford University Press, Oxford, 2000, pp. 218.

Application

Applications of Fmoc-Cys(Trt)-NovaSyn^® TGT include:

• the preparation of a phosphoprotein using Native Chemical Ligation (NCL).
• the preparation of a peptide intermediate for use in glycoprotein semi-synthesis.

Other Notes

Replaces: 04-12-2705

Legal Information

NOVASYN is a registered trademark of Merck KGaA, Darmstadt, Germany

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable