407654

Interleukin-6, Mouse, Recombinant, E. coli

• Synonym(s): rmIL-6
• NACRES: NA.41
• UNSPSC Code: 12352202
• Form: lyophilized
• Assay: ≥97% (SDS-PAGE)

Properties

• assay: ≥97% (SDS-PAGE)
• Quality Segment: 100
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤1 EU/μg Endotoxin (EU/μg IL-6)
• shipped in: ambient
• storage temp.: −70°C

Description

General description

Recombinant, mouse interleukin-6 expressed in E. coli. Stimulates the production of acute phase proteins by hepatocytes. Induces growth and differentiation of B cells, hepatocytes, keratinocytes, and nerve cells. Does not inhibit any significant activity on human cells.

Biochem/physiol Actions

ED₅₀ = 20-60 pg/ml as measured in a cell proliferation assay with a factor-dependent plasmacytoma cell line

Physical form

Lyophilized from sterile-filtered 50 mM NaOAc, 1 mM EDTA, 5% trehalose, 50 µg BSA/µg IL-6 pH 4.0.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C or -70°C or for up to 1 month at 4°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Other Notes

Matsuda, T., et al. 1994. Biochem. Biophys. Res. Commun.202, 637.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable