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| Size/SKU | Availability | Price |
|---|---|---|
500 U | Estimated to ship onMay 25, 2026fromKuehne + Nagel Sp. z o.o. | PLN 1,700.00 |
About This Item
NACRES:
NA.54
UNSPSC Code:
12352202
Form:
liquid
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Segment
form
liquid
specific activity
≥10,000 units/mL, ≥5,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
technique(s)
molecular cloning: suitable
input
purified RNA
foreign activity
Nuclease-free
shipped in
wet ice
storage temp.
−20°C
General description
Recombinant HIV reverse transcriptase expressed in E. coli. Catalyzes error-prone synthesis on DNA and RNA templates. Provides an excellent target for evaluating antiviral agents.
Application
HIV Reverse Transcriptase, Recombinant, E.coli has been used as a standard in reverse transcriptase activity assay.[1]
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 10 mM potassium phosphate, 1 mM DTT, 20% glycerol, pH 7.4.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
One unit is defined as the amount of enzyme that will incorporate 1.0 nmol of labeled dTTP into acid-insoluble material in 20 min at 37°C, pH 8.3.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
1 of 1
This Item | |||
|---|---|---|---|
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| technique(s) molecular cloning: suitable | technique(s) - | technique(s) - | technique(s) - |
| form liquid | form liquid | form liquid | form liquid |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −70°C | storage temp. −20°C | storage temp. −70°C |
| specific activity ≥10,000 units/mL, ≥5,000 units/mg protein | specific activity - | specific activity - | specific activity ≥300,000 U/mg, ≥50,000 units/mL |
Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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