324696

Methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide

A specific chromogenic substrate for human neutrophil elastase.

• Synonym(s): Elastase Substrate I, Colorimetric
• Empirical Formula (Hill Notation): C₂₇H₃₈N₆O₉
• CAS Number: 70967-90-7
• Molecular Weight: 590.63
• UNSPSC Code: 12352200
• NACRES: NA.54
• MDL number: MFCD00077137
• Assay: ≥95% (HPLC)
• Form: lyophilized
• Storage condition: OK to freeze, desiccated

Properties

• Product Name: Elastase Substrate I, Colorimetric, A specific chromogenic substrate for human neutrophil elastase.
• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated
• solubility: water: 1 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: [N+](=O)([O-])c1ccc(cc1)N[C@@H](C(C)C)C(=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)CCC(=O)OC)C)C
• InChI: 1S/C27H38N6O9/c1-15(2)23(30-18-8-10-19(11-9-18)33(40)41)26(38)31-25(37)20-7-6-14-32(20)27(39)17(4)29-24(36)16(3)28-21(34)12-13-22(35)42-5/h8-11,15-17,20,23,30H,6-7,12-14H2,1-5H3,(H,28,34)(H,29,36)(H,31,37,38)/t16-,17-,20-,23-/m0/s1
• InChI key: JWYKDZIEIWGSBN-OSAZLGQLSA-N

Description

General description

A specific chromogenic substrate for human neutrophil elastase (K_cat/K_m = 120,000 vs. 2700 for pancreatic elastase). Cleavage can be monitored at ~410 nm. Sold on the basis of substrate content.

Specific chromogenic substrate for human neutrophil elastase (k_cat/K_m = 120,000 versus pancreatic elastase: k_cat/K_m = 2,700). Cleavage can be monitored at 410 nm using ε = 8,800.

Biochem/physiol Actions

Cell permeable: no

Kcat/Km = 120,000 vs. 2700 for pancreatic elastase

Primary Target
Chromogenic substrate for human neutrophil elastase

Product does not compete with ATP.

Reversible: no

Physical form

Lyophilized from a solution containing 90 mg D-mannitol and 10 mg substrate.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Barrett, A.J., et al. 1989. Biochim. J.260, 259.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

MeOSuc-Ala-Ala-Pro-Val-pNA

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable