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For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
324626
EGTA, Molecular Biology Grade
Synonym(s):
EGTA, Molecular Biology Grade, Ethyleneglycol- bis(β-aminoethyl)-N,N,Nʹ,Nʹ-tetraacetic Acid
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About This Item
Recommended Products
grade
Molecular Biology
Quality Level
Assay
≥98% dry basis (complexometric)
form
solid (fine)
reaction suitability
reagent type: chelator
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
solubility
1 M NaOH: soluble
cation traces
heavy metals (as Pb): ≤0.001%
foreign activity
DNases, proteases, RNases, none detected
shipped in
ambient
storage temp.
10-30°C
SMILES string
N(CCOCCOCCN(CC(=O)O)CC(=O)O)(CC(=O)O)CC(=O)O
InChI
1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
InChI key
DEFVIWRASFVYLL-UHFFFAOYSA-N
General description
Application
- Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.: This study presents a genetically encoded biosensor for real-time monitoring of lysosomal pH changes, potentially enhanced by buffering agents like EGTA. The findings may offer insights into cellular mechanisms influenced by calcium chelation (Ponsford et al., 2021).
- Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca(2+).: The research highlights the role of calcium in fungal development, with EGTA potentially influencing these biological processes through calcium chelation (Zhu et al., 2019).
Warning
Reconstitution
Other Notes
Bers, D.M. 1982. Am. J. Physiol.242, C404.
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Certificates of Analysis (COA)
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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