17-218
Ras Activation Assay Kit
Synonym(s):
Ras Activity Assay
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About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Recommended Products
manufacturer/tradename
Upstate®
Quality Level
technique(s)
activity assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... MRAS(22808)
General description
Muscle RAS oncogene homolog (MRAS) plays a key role in cardiovascular diseases. The MRAS gene codes for a member of the ras superfamily of GTP-binding proteins. MRAS is found in all tissues, particularly in the cardiovascular system. It is involved in signal transduction, including cell growth and differentiation.
Packaging
Kit capacity: 30 assays
Components
Ras Assay Reagent (Raf-1 RBD, agarose) (Cat.# 14-278)
Anti-Ras, clone RAS10 (Cat.# 05-516)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
Anti-Ras, clone RAS10 (Cat.# 05-516)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
Quality
This kit precipitated Ras-GTP from HeLa cell lysates that had been loaded with GTPγS. The precipitated Ras-GTP was detected by western immunoblot using 1μg/ml anti-Ras, clone RAS10
Linkage
Replaces: SGT435
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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AnhCo Nguyen et al.
Molecular and cellular biology, 22(9), 3035-3045 (2002-04-10)
While scaffold proteins are thought to be key components of signaling pathways, their exact function is unknown. By preassembling multiple components of signaling cascades, scaffolds are predicted to influence the efficiency and/or specificity of signaling events. Here we analyze a
Richard J Calvert et al.
Analytical biochemistry, 343(2), 283-292 (2005-07-16)
Accurate measurement of activity of wild-type K-ras protein is important due to its tumor suppressor action in tissues such as lung. A published method by Taylor and co-workers uses plasmid-containing Escherichia coli cells to produce a glutathione-S-transferase/raf-1 ras binding domain
X Shen et al.
The Biochemical journal, 352 Pt 3, 913-919 (2000-12-06)
Interleukin 1beta (IL-1beta) suppresses the IL-6-dependent induction of type II acute-phase response genes, but the underlying mechanism for this suppression remains uncertain. Here we report that treatment of human hepatocullular carcinoma HepG2 cells with IL-1beta inhibited the IL-6-dependent binding of
Michael R Bennett et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 23(18), 7207-7217 (2003-08-09)
Neurofibromatosis type 1 (NF1) patients are predisposed to learning disabilities, macrocephaly, and brain tumors as well as abnormalities on magnetic resonance imaging that are postulated to result from abnormal myelination. Here we show that Nf1+/- spinal cords in adult mice
J Kim et al.
The Journal of biological chemistry, 276(22), 19102-19110 (2001-03-23)
AMP-activated protein kinase (AMPK) is tightly regulated by the cellular AMP:ATP ratio and plays a central role in the regulation of energy homeostasis. Previously, AMPK was reported to phosphorylate serine 621 of Raf-1 in vitro. In the present study, we
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