While this antibody hasn't been validated for flow cytometry, independent laboratories have published its use for this application. For example:
https://www.nature.com/articles/s42003-020-1072-4.pdf
F1804
ANTI-FLAG® M2 antibody, Mouse monoclonal
1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
ANTI-FLAG® M2 antibody, Mouse monoclonal
Synonym(s):
flag antibody, Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2
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| Size/SKU | Availability | Price |
|---|---|---|
50 μg | Please contact Customer Service for Availability | PLN 674.00 |
200 μg | Please contact Customer Service for Availability | PLN 2,750.00 |
1 mg | Please contact Customer Service for Availability | PLN 5,370.00 |
5 mg | Please contact Customer Service for Availability | PLN 8,120.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
M2, monoclonal
Application:
—
Citations:
9676
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
purified by
affinity chromatography (affinity tag purification)
species reactivity
all
concentration
1.0-1.2 mg/mL
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
wet ice
storage temp.
−20°C
General description
The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.
Method of purification - Affinity tag purification
Method of purification - Affinity tag purification
Immunogen
FLAG; peptide sequence DYKDDDDK
Application
For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) [1], immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Western Blotting and EIA
Learn more product details in our FLAG® application portal.
Biochem/physiol Actions
Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C
Physical form
Contains 50% glycerol in phosphate buffered saline.
Preparation Note
Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
This Item | |||
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form - | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| biological source mouse | biological source - | biological source mouse | biological source - |
| conjugate unconjugated | conjugate - | conjugate biotin conjugate | conjugate agarose conjugate |
| clone M2, monoclonal | clone M2, monoclonal | clone M2, monoclonal | clone M2, monoclonal |
| species reactivity all | species reactivity - | species reactivity all | species reactivity - |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in wet ice |
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flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
10 - Combustible liquids
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Can this Antibody be used for Flow cytometry?
1 answer-
Helpful?
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Any protocol available for using the Anti-Flag M2 (F1804) in a ChIP-Seq assay? And, if the lysis buffer contains SDS and sodium deoxycholate at a concentration of 0.1%, will the presence of both denature or interfere with binding in the research process?
1 answer-
Several papers have cited F1804 in ChIP & ChIPseq applications:
https://www.nature.com/articles/s41467-023-40803-y.pdf
https://www.nature.com/articles/s41467-023-41307-5.pdf
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10541012&blobtype=pdf
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10541012&blobtype=pdf
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10693086&blobtype=pdf
https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10863984&blobtype=pdfAnti-FLAG® M2 Magnetic Beads are not compatible with deoxycholate and SDS. Please refer to compatibility chart on the datasheet linked below for further information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/075/m8823bul-ms.pdfHelpful?
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Is this Ab suitable for ELISA?
1 answer-
This antibody is compatible with enzyme immunoassay, which includes ELISA, the most widely used form of EIA.
Helpful?
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Bonjour, il est indiqué que cet anticorps marche en immunohistochimie. Quel protocole d'antigen retrieval faut-il utiliser et quelle concentration d'anticorps sur coupe FFPE? Merci
1 answer-
Please see the reference at the link below using this antibody at 1:50 dilution in IHC with antigen retrieval in citrate buffer for 5 minutes.
https://www.nature.com/articles/s41467-019-12187-5.pdfHelpful?
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モノクローナル抗FLAG® M2抗体 マウス宿主抗体(F1804)を購入しました。WBで使用しますが、抗体の推奨の希釈率、希釈液を知りたいです。よろしくお願いいたします。
1 answer-
An antibody concentration of 1 ug/mL or 1:1000 dilution is recommended for use in western blot. Please see the link below to review the product datasheet which includes the full protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdfHelpful?
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