16-232

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor^™ 488 conjugate

clone DM1A, Upstate^®, from mouse

• Synonym(s): Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, tubulin, alpha 1a, tubulin, alpha 3, tubulin, alpha, brain-specific
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: ALEXA FLUOR^™ 488
• Clone: DM1A, monoclonal
• Application: FACS, ICC, IF, WB
• Citations: 17

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: ALEXA FLUOR^™ 488
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: DM1A, monoclonal
• species reactivity: rat, mouse, guinea pig, human, pig, bovine, avian
• manufacturer/tradename: Upstate^®
• technique(s): flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NP_006000
• UniProt accession no.: Q71U36
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TUBA1A(7846)

Description

General description

50-60 kDa

Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.

Immunogen

Epitope: The epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).

Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).

Application

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.

Western Blot Analysis:
2 µg/mL of a previous lot detected α-Tubulin in RIPA lysates from A431 cells.

Immunocytochemistry:
2 µg/mL of a previous lot showed positive immunostaining for α-Tubulin in HeLa cells.

Biochem/physiol Actions

Recognizes α-Tubulin, clone DM1A, Mr 50-60 kDa.

Physical form

Alexa Fluor^™ 488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween-20, 0.05% sodium azide.
Liquid at 2-8°C.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.
Do Not Freeze. Do not store the material diluted.
For maximum recovery of product, centrifuge original vial prior to removing cap.

Analysis Note

Control
Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor^™ 488-conjugate.

Routinely evaluated by Flow Cytometry.

Flow Cytometry:
0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable