Skip to Content
Merck

Skip To

1.52258

Chromolith® WP 300 Protein A (monolithic) HPLC Columns

L × I.D. 25 mm x 4.6 mm HPLC Column

Synonym(s):

Chromolith® WP 300 Protein A 25-4.6 HPLC column

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
1 unit
Please contact Customer Service for Availability
PLN 6,360.00

About This Item

UNSPSC Code:
41115700
NACRES:
SB.52
Matrix active group:
Protein A bonding phase
Pore size:
2 μm macroporosity, 30 nm mesoporosity
Matrix:
monolithic silica

PLN 6,360.00


Please contact Customer Service for Availability

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


Product Name

Chromolith® WP 300 Protein A, L × I.D. 25 mm x 4.6 mm HPLC column

material

PEEK column

product line

Chromolith®

feature

endcapped: no

packaging

1 ea of

parameter

2 - 45 °C temp. range, 2-45 °C temp. range, 200 bar pressure

technique(s)

HPLC: suitable, LC/MS: suitable

column L × I.D.

2.5 cm × 4.6 mm

surface area

120 m2/g

matrix

monolithic silica

matrix active group

Protein A bonding phase

pore size

2 μm macroporosity, 30 nm mesoporosity

operating pH range

1.5-7.5

operating pH

1.5-7.5

separation technique

affinity

storage temp.

2-8°C

General description

Affinity chromatography is a selective technique which takes advantage of specific molecular interactions, for example antigen and antibody. The Chromolith® WP 300 Protein A HPLC column is designed to monitor monoclonal antibody titer and yield determination from cell-culture supernatants. This analytical scale procedure helps to optimize the titer of monoclonal antibody for the optimal time for harvest of the monoclonal antibody products.

Chromolith® WP 300 Protein A 25-4.6 columns are made of monolithic silica with a characteristic bimodal pore structure.

Key Benefits:
—Rapid and accurate titer determination across a wide range of injection volumes
—Consistent binding efficiencies even at high flow rates
—Expanded column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

Chromolith® WP 300 Protein A is available in 2 mm I.D. providing improved efficiency, sensitivity, and enhanced MS-compatibility.
Flyer: Rapid and Robust Antibody Quantitation - Chromolith® WP 300 Protein A HPLC columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
1.522701.523581.52265
separation technique

affinity

separation technique

reversed phase

separation technique

-

separation technique

reversed phase

matrix active group

Protein A bonding phase

matrix active group

C18 bonding phase

matrix active group

Protein A bonding phase

matrix active group

C8 bonding phase

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)

pore size

2 μm macroporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 mm mesoporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 nm mesoporosity

pore size

2 μm macroporosity, 30 nm mesoporosity

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

matrix

monolithic silica

product line

Chromolith®

product line

Chromolith®

product line

-

product line

Chromolith®


Still not finding the right product?

Explore all of our products under Chromolith® WP 300 Protein A




Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Questions

Reviews

No rating value

Active Filters