09-258

Anti-phospho-PAK1 (Ser199/Ser204) Antibody

Upstate^®, from rabbit

• Synonym(s): Anti-IDDMSSD, Anti-PAKalpha, Anti-alpha-PAK, Anti-p65-PAK
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human
• Application: WB
• Citations: 6

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse (100% immunogen homology), rat (100% immunogen homology)
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• NCBI accession no.: NP_002567
• UniProt accession no.: Q13153
• shipped in: dry ice
• target post-translational modification: phosphorylation (pSer199/pSer204)
• Gene Information: human ... PAK1(5058) ; mouse ... Pak1(18479) ; rat ... Pak1(29431)

Description

General description

65 kDa

p21-activated kinases (PAK) are Ser/Thr kinases, which are activated by, and are effectors of Rho family of GTPases. As such, they are implicated in Actin polymerization and activation of the stress-activated kinase cascades. PAK1 is phosphorylated by cdk5, resulting in its inhibition. PAK2 can be activated by Caspase-cleavage in addition to p21-binding, implicating it in cytoskeletal changes during apoptosis. PAK3 is expressed at high levels in post-mitotic neurons of the developing post-natal cerebral cortex and hippocampus, and is the mutant gene thought responsible for non-syndromic X-linked mental retardation.

Immunogen

Amino Acids encompassing and including phosphorylated Ser199 and Ser204 of human PAK1. Sequence is highly homologous (greater than 90%) to PAK2 and PAK3.

Epitope: Ser199/Ser204 (Central)

Application

Detect phospho-PAK1 (Ser199/Ser204) using this Anti-phospho-PAK1 (Ser199/Ser204) Antibody validated for use in WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Recognizes PAK1 phosphorylated on Ser199 and Ser204 (human)/Ser198 and Ser203 (mouse). Immunogen sequence is higly homolgous (greater than 90%) with PAK2 and PAK3.

Physical form

Affinity purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol

Double affinity purification over both non-phospho-peptide column followed by phopshorylated peptide column.

Preparation Note

Stable for 1 year at -20°C from date of receipt.

Analysis Note

Routinely evaluated by western blot on untreated and pervanadate-treated Jurkat cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable