07-1387

Anti-oxidized-CaM Kinase II (Met281/282) Antibody

serum, from rabbit

• Synonym(s): CAMKK beta protein, CaM-KK beta, CaM-kinase kinase beta, CaMKK beta, Calcium/calmodulin-dependent protein kinase kinase beta, calcium/calmodulin-dependent protein kinase beta, calcium/calmodulin-dependent protein kinase kinase 2 beta, calcium/calmodulin-
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 19

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, rat, human
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_742127
• UniProt accession no.: Q96RR4
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CAMK2D(817)

Description

General description

The Ca2+/Calmodulin-Dependent Kinase family includes CaM Kinases I through IV, Phosphorylase Kinase, and MLCK. The CaM Kinases are inactive in the absence of stimuli; binding to Ca2+/Calmodulin induces conformational changes and activation. CaM Kinases have broad substrate specificity, with many cellular functions.

60 kDa

Immunogen

Epitope: Oxidized Met281/282

Synthetic peptide encompassing and including oxidized Met281/282.

Application

An independent lab validated this antibody to work in immunocytochemistry in heart sections from mice treated with saline, AngII,or Iso (Erickson, J.R., 2008).

This Anti-oxidized-CaM Kinase II (Met281/282) Antibody is validated for use in WB, IC for the detection of oxidized-CaM Kinase II (Met281/282).

Biochem/physiol Actions

Expected to cross-react on Mouse and Rat based on 100% homology with immunogen sequence.

This antibody recognizes CaM Kinase II when oxidized on Met281/282.

Preparation Note

Stable for 1 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Analysis Note

Evaluated by western blot on untreated and H2O2-treated mouse heart cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect oxidized CaM Kinase II in mouse heart cell lysates.

Other Notes

Replaces: 04-1079

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1