06-935

Anti-Estrogen Receptor α Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, IHC, IP, WB
• Citations: 152

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), rat (immunogen homology)
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_036821
• UniProt accession no.: P06211
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ESR1(2099) ; mouse ... Esr1(13982) ; rat ... Esr1(24890)

Description

General description

Estrogen receptor (UniProt: P06211; also known as ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1) is encoded by the Esr1 (also known as Esr, Estr, Nr3a1) gene (Gene ID: 24890) in rat. Estrogen receptor α is a member of the steroid family of nuclear receptors; a critical nuclear receptor that mediates the physiological effects of estrogens. Estrogen receptor α contains several functional domains, including a ligand-binding domain, DNA-binding domain, and activation functions (AF-1 and AF-2), which regulate transcriptional activity. It is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor α can positively or negatively regulate gene transcription. Estrogen receptor α is expressed in various tissues, including the brain, breast, liver, the cardiovascular system, uterus, and placenta, where it plays roles in development, reproduction, and metabolic regulation. Estrogen receptor α both a nuclear receptor as well as a membrane-associated receptor through post-translational modifications like palmitoylation. This dual localization enables Estrogen receptor α to participate in both genomic and non-genomic signaling pathways, such as interactions with the PI3K/AKT and MAPK/ERK pathways, and activation of endothelial nitric oxide synthase (eNOS). Protein-protein interactions involving Estrogen receptor α include binding to transcription factors like AP-1 and SP-1, as well as interaction with caveolin-1 at the membrane. Dysregulation of Estrogen receptor α signaling is implicated in various diseases, including hormone-dependent cancers (e.g., breast cancer) and metabolic disorders. (Ref.: Ge, Y. et al., (2023). Oncology Letters, 26, 530).

Target molecular weight ~ 58 kDa observed. 67.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of rat Estrogen Receptor α.

Epitope: C-terminus.

Application

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Estrogen receptor α in MCF7 cell lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Estrogen Receptor α in MCF7 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Estrogen receptor α in human breast cancer and uterine cancer tissue sections and a 1:500 dilution in human cervical cancer tissue sections.

Immunoprecipitation Analysis: 4 µg from a representative lot immunoprecipitated Estrogen receptor α from MCF7 cell lysate.


Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Anti-Estrogen Receptor , Cat. No. 06-935, is a rabbit polyclonal antibody that detects Estrogen Receptor and is tested for use in Western Blotting, Immunocytochemistry, Immunoprecipitation, and Immunohistochemistry (Paraffin).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Hormones & Receptors

Biochem/physiol Actions

This rabbit polyclonal antibody detects rat Estrogen Receptor α. It targets an epitope within 15 amino acids from the C-terminal region.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity purified

Preparation Note

Recommended storage: +2°C to +8°C.

Analysis Note

Control
MCF7 cell lysate

Evaluated by Western Blotting in MCF-7 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Estrogen Receptor in MCF-7 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable