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Anti-Nck Antibody

Name: Anti-Nck Antibody
Brand: MM
Price: 1960 PLN
Availability: InStock

Upstate^®, from rabbit

Synonym(s):

Anti-NCK, Anti-nck-1

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Pack Size	SKU	Availability	Price
100 μg		Estimated to ship onMay 19, 2026fromKuehne + Nagel Sp. z o.o.	PLN 1,960.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IP, WB

Citations:

PLN 1,960.00

Estimated to ship onMay 19, 2026Details

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Properties

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Upstate^®

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG

NCBI accession no.

NM_010878

UniProt accession no.

P16333

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NCK1(4690)

Description

General description

47kDa

Immunogen

Purified GST fusion protein produced in E. coli., corresponding to human Nck amino acid sequence 1 to 254

Application

Detect Nck using this Anti-Nck Antibody validated for use in IP & WB.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Nck

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing 0.05% sodium azide

Format: Purified

Protein G Chromatography

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on human A431 carcinoma cells, mouse 3T3/A31 fibroblasts or rat L6 skeletal fibroblasts

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Show Differences

1 of 1

This Item	MAB356	SAB1412681	HPA030766
Sigma-Aldrich 06-288 Anti-Nck Antibody	Sigma-Aldrich MAB356 Anti-Substance P Antibody, pain, clone NC1	Sigma-Aldrich SAB1412681 ANTI-NCK1 antibody produced in mouse	Sigma-Aldrich HPA030766 Anti-NCK1 antibody produced in rabbit
biological source rabbit	biological source rat	biological source mouse	biological source rabbit
species reactivity mouse, rat, human	species reactivity human	species reactivity human	species reactivity human
clone polyclonal	clone NC1, monoclonal	clone polyclonal	clone polyclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form purified antibody	antibody form culture supernatant	antibody form purified immunoglobulin	antibody form affinity isolated antibody
UniProt accession no. P16333	UniProt accession no. P20366	UniProt accession no. P16333	UniProt accession no. P16333

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Independent SH2-binding sites mediate interaction of Dok-related protein with RasGTPase-activating protein and Nck.

P Lock et al.

The Journal of biological chemistry, 274(32), 22775-22784 (1999-07-31)

A murine embryonic cDNA library was screened for potential substrates of the Src family kinase, Lyn, using a phosphorylation-screening strategy. One cDNA that we identified encodes Dok-related protein (DokR), a protein with homology to p62(dok) (Dok), and members of the

Src homology region 2 domain-containing phosphatase 1 positively regulates B cell receptor-induced apoptosis by modulating association of B cell linker protein with Nck and activation of c-Jun NH2-terminal kinase.

Kazuya Mizuno et al.

Journal of immunology (Baltimore, Md. : 1950), 169(2), 778-786 (2002-07-05)

Src homology region 2 domain-containing phosphatase 1 (SHP-1) is a key mediator in lymphocyte differentiation, proliferation, and activation. We previously showed that B cell linker protein (BLNK) is a physiological substrate of SHP-1 and that B cell receptor (BCR)-induced activation

Characterization of Cbl-Nck and Nck-Pak1 interactions in myeloid FcgammaRII signaling.

K D Izadi et al.

Experimental cell research, 245(2), 330-342 (1998-12-16)

Fc receptors modulate inflammatory processes, including phagocytosis, serotonin and histamine release, superoxide production, and secretion of cytokines. Aggregation of FcgammaRIIa, the low-affinity receptor for monomeric IgG, activates nonreceptor protein tyrosine kinases such as Lyn, Hck, and Syk, potentially driving the