04-773

Anti-phospho-MYPT1 (Thr850) Antibody,, rabbit monoclonal

culture supernatant, clone SA19, from rabbit

• Synonym(s): Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, myosin phosphatase, target subunit 1, protein phosphatase 1, regulatory (inhibitor) subunit 12A
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: SA19, monoclonal
• Species reactivity: rat, chicken, mouse, human
• Application: WB
• Citations: 5

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: SA19, monoclonal
• species reactivity: rat, chicken, mouse, human
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_002471.1
• UniProt accession no.: O14974
• shipped in: dry ice
• target post-translational modification: phosphorylation (pThr850)
• Gene Information: human ... PPP1R12A(4659)

Description

General description

Doublet at ~130 kDa

Phosphorylation of myosin is crucial to smooth muscle contraction. The degree of myosin phosphorylation is dependent in part by the activity of myosin phosphatase. Recent findings suggest that this enzyme can be regulated, which has lead to interest in characterizing this phosphatase. Myosin phosphatase seems to be comprised of three subunits (catalytic subunit of type 1 phosphatase-δ (PP1) and two non-catalytic subunits, large and small (M20)). Studies suggest that the large subunit is a targeting subunit and is termed myosin phosphatase target subunit (MYPT). MYPT has been shown to be a substrate for the Rho-associated kinase (ROK). ROK mediates an agonist-induced increase in myosin phosphorylation and force by inhibiting myosin light chain phosphatase activity through phosphorylation of MYPT.

Immunogen

Epitope: pThr850(Ch) or pThr853(H)

KLH-conjugated, synthetic peptide containing the sequence …RS[pT]GV…, in which [pT] corresponds to phosphorylated threonine at amino acid residue 850 of chicken myosin phosphatase target subunit 1 (MYPT1). Corresponds to Thr853 in human MYPT1.

Application

Anti-phospho-MYPT1 (Thr850) Antibody, detects level of phospho-MYPT1 (Thr850) & has been published & validated for use in WB.

Biochem/physiol Actions

Predicted to cross-react with mouse and rat.

Recognizes MYPT1 phosphorylated on Thr850, doublet at Mr ~130 kDa. A non-specific protein was also detected, Mr ~55-60 kDa.

Analysis Note

Control
HEK293 cell lysate phosphorylated by ROKα/ROCKII.

Westerin Blot Analysis: A 1:500-1:1000 dilution of this antibody detected MYPT1 in RIPA lysates from HEK293 cells phosphorylated by preincubation with ROKα/ROCK-II, active (Catalog # 14-338).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 05-773

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable