04-147

Anti-ASC Antibody, clone 2EI-7

clone 2EI-7, from mouse

• Synonym(s): Caspase recruitment domain-containing protein 5, PYD and CARD domain containing, PYD and CARD domain-containing protein, Target of methylation-induced silencing 1, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain prot
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 2EI-7, monoclonal
• Application: FACS, WB
• Citations: 38

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2EI-7, monoclonal
• species reactivity: mouse
• species reactivity (predicted by homology): human (based on 100% sequence homology)
• technique(s): flow cytometry: suitable, western blot: suitable
• isotype: IgG1κ

Description

General description

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, Bcl10, Apaf-1, ARC, caspase-2 and caspase-9. A novel CARD domain containing protein has been identified in human and mouse and designated ASC and TMS1. Irregular expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells. Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.

~22 kDa

Immunogen

Epitope: Unknown

Full length recombinant human ASC.

Application

Detect ASC using this Anti-ASC Antibody, clone 2EI-7 validated for use in WB, FC.

Research Category
Inflammation & Immunology

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

This antibody recognizes ASC.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse spleen tissue lysate

Evaluated by Western Blot on mouse spleen tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected ASC in mouse spleen tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1