03-312M

Magna ChIRP Human HOTAIR lncRNA Probe Set

The Magna ChIRP Human HOTAIR lncRNA Probe Set contains 48 predesigned 20-mer DNA oligonucleotides tiled along and complementary to the sequence of Human lncRNA HOTAIR.

• UNSPSC Code: 12161503
• NACRES: NA.77
• eCl@ss: 32161000

Properties

• Quality Segment: 100
• manufacturer/tradename: Chemicon^®, Magna ChIRP^®
• technique(s): DNA sequencing: suitable, RNA extraction: suitable (nuclear function kit), RNA purification: suitable (with magnetic beads), activity assay: suitable (protein interaction), protein purification: suitable (protein determination)

Description

General description

Pervasive genomic transcription and the role of non-coding RNAs in this process is a rapidly evolving field that remains to be thoroughly explored in gene regulation.
Historically, chromatin immunoprecipitation (ChIP) has been used to interrogate association of proteins with genomic DNA sequences. Given that chromatin contains not only DNA and DNA-binding proteins, but also RNA-binding proteins and RNA, new methods have been developed to map the association of these RNA molecules to distinct regions of the genome. One such method, designated ChIRP (Chromatin Isolation by RNA Purification), allows recovery of specific segments of DNA and proteins using chromatin associated RNA as a target. This unique approach allows isolation of chromatin-associated RNAs (e.g. lncRNAs) within specific regions of genomic DNA sequences, as well as proteomic analysis of protein components of the lncRNA regulatory complexes. The Magna ChIRP RNA Interactome Kits (cat. # 17-10494 and 17-10495) facilitate the use of this method and has been proven compatible with this probe set.

Physical form

10 mM Tris-HCl, 1mM EDTA, pH 8.0

Preparation Note

Upon receipt store at -20°C. Avoid multiple freeze thaws. With proper storage and handling this product is stable for 6 months from the date of receipt.

Analysis Note

Verified by Electrospray Ionization Mass Spectrometry. Final yields are determined using UV absorbance at OD260. Concentrations are determined using UV absorbance at OD260 before pooling. Oligos will manufactured under ISO 9001 conditions.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

MAGNA CHIRP is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids