E45260
Woodward′s reagent K
95%
Synonym(s):
2-Ethyl-5-phenylisoxazolium-3′-sulfonate, NEPIS
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About This Item
Empirical Formula (Hill Notation):
C11H11NO4S
CAS Number:
Molecular Weight:
253.27
Beilstein:
4149224
EC Number:
MDL number:
UNSPSC Code:
12352005
PubChem Substance ID:
NACRES:
NA.22
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Assay
95%
reaction suitability
reaction type: Coupling Reactions
mp
220 °C (dec.) (lit.)
application(s)
peptide synthesis
SMILES string
CC[n+]1ccc(o1)-c2cccc(c2)S([O-])(=O)=O
InChI
1S/C11H11NO4S/c1-2-12-7-6-11(16-12)9-4-3-5-10(8-9)17(13,14)15/h3-8H,2H2,1H3
InChI key
MWOOKDULMBMMPN-UHFFFAOYSA-N
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Jongchan Woo et al.
Protein science : a publication of the Protein Society, 17(4), 725-735 (2008-03-25)
Renilla luciferase (RLUC) is a versatile tool for gene expression assays and in vivo biosensor applications, but its catalytic mechanism remains to be elucidated. RLUC is evolutionarily related to the alpha/beta hydrolase family. Its closest known homologs are bacterial dehalogenases
Hassan Faridnouri et al.
Bioelectrochemistry (Amsterdam, Netherlands), 82(1), 1-9 (2011-07-01)
This work describes the reaction mechanism for chemical modification of tyrosinase by Woodward's Reagent K and its covalent attachment to a glassy carbon electrode. The spectrophotometric studies revealed that the modification does not cause a significant structural change to tyrosinase.
P Bustos et al.
Journal of protein chemistry, 15(5), 467-472 (1996-07-01)
The reaction of Woordward's reagent K (WRK) with model amino acids and proteins has been analyzed. Our results indicate that WRK forms 340-nm-absorbing adducts with sulfhydryl- and imidazol-containing compounds, but not with carboxylic acid derivatives, in agreement with Liamas et
E Winkler et al.
Biochemistry, 36(1), 148-155 (1997-01-07)
The transport inhibiting nucleotide binding to the uncoupling protein (UCP) has a unique pH dependence and has been postulated to be controlled by the dissociation state of a carboxyl group in UCP with pK 4.5 and, in addition only for
Nelson Carvajal et al.
The protein journal, 23(3), 179-183 (2004-06-18)
Human liver arginase (EC 3.5.3.1) was totally inactivated by incubation with Woodward's reagent K (WRK). The inactivation followed pseudo-first-order kinetics, and the order of the inactivation was close to 1, consistent with reaction of one molecule of WRK with one
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