283924

Rhodamine B isothiocyanate

mixed isomers

• Synonym(s): RBITC
• Empirical Formula (Hill Notation): C₂₉H₃₀ClN₃O₃S
• CAS Number: 36877-69-7
• Molecular Weight: 536.08
• EC Number: 253-248-9
• MDL number: MFCD00136007
• UNSPSC Code: 12171500
• PubChem Substance ID: 329753185
• NACRES: NA.47

Properties

• form: powder
• color: purple to violet; red to red-brown
• solubility: methanol: 10 mg/mL
• λmax: 555 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1
• InChI: 1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H
• InChI key: YVSWPCCVTYEEHG-UHFFFAOYSA-N

Description

General description

Rhodamine B isothiocyanate (RBITC) is a fluorescent dye. It acts as an anterograde and retrograde marker of retinal neurons in the adult rat. (current GD)It is also known as RBITC, RhIc, RITC, and TRITC.

Application

Rhodamine B isothiocyanate has been used as a fluorescent probe to label the pathogenic bacterium Staphylococcus aureus and is also used to label type I collagen (C1). Other applications include -

• labeling immunoglobulins
• as a fluorescent probe of living cells
• RBITC-Iabeled molecules like dextrans have been applied as probes of a cell′s endocytic activity and to mark cells to allow their subsequent migration to be detected

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H302 + H312 + H332,H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves