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apHera™ NH₂ (5 μm) HPLC Columns

L × I.D. 1 cm × 4.6 mm, HPLC Guard Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

apHera™ NH2 HPLC Guard Column, 5 μm particle size, L × I.D. 1 cm × 4.6 mm

material

stainless steel column

Agency

suitable for USP L82

product line

apHera™

packaging

pkg of 1 ea

manufacturer/tradename

apHera™

parameter

147 bar pressure (2133 psi)
4-50 °C temperature

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

1 cm × 4.6 mm

matrix

polymeric

matrix active group

amino phase

particle size

5 μm

pore size

300 Å

operating pH

1-14

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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apHera is a trademark of Sigma-Aldrich Co. LLC

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Khin Than Myint et al.
Analytical chemistry, 81(18), 7766-7772 (2009-08-26)
We have developed a practical method for the comprehensive analysis of polar anionic metabolites in biological samples with the use of a nano-LC/MS system. A polyamine-bonded polymer-based apHera NH2 column, which is compatible with ammonium carbonate buffer, effectively retained anionic
Marine de Person et al.
Journal of chromatography. A, 1081(2), 174-181 (2005-07-26)
A new procedure based on hydrophilic interaction chromatography coupled to tandem mass spectrometry (ionisation process by pneumatically assisted electrospray in negative ion mode), is developed and validated for the simultaneous determination of underivatised taurine and methionine in beverages rich in

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