56403AST
apHera™ NH₂ (5 μm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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product name
apHera™ NH2 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
Agency
suitable for USP L82
product line
apHera™
packaging
pkg of 1 ea
manufacturer/tradename
apHera™
parameter
147 bar pressure (2133 psi)
4-50 °C temperature
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
matrix
polymeric
matrix active group
amino phase
particle size
5 μm
pore size
300 Å
operating pH
1-14
application(s)
food and beverages
separation technique
hydrophilic interaction (HILIC)
normal phase
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General description
apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.
Features of Amino apHera™ Columns
Features of Amino apHera™ Columns
- High efficiency for carbohydrate analysis
- Ideal for basic conditions/amphoteric detection
- High selectivity mono- to oligosaccharides
- pH range 2-13
- 300 angstrom pore size
- Stable, predictable retention
- Long column life
Legal Information
apHera is a trademark of Sigma-Aldrich Co. LLC
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Khin Than Myint et al.
Analytical chemistry, 81(18), 7766-7772 (2009-08-26)
We have developed a practical method for the comprehensive analysis of polar anionic metabolites in biological samples with the use of a nano-LC/MS system. A polyamine-bonded polymer-based apHera NH2 column, which is compatible with ammonium carbonate buffer, effectively retained anionic
Marine de Person et al.
Journal of chromatography. A, 1081(2), 174-181 (2005-07-26)
A new procedure based on hydrophilic interaction chromatography coupled to tandem mass spectrometry (ionisation process by pneumatically assisted electrospray in negative ion mode), is developed and validated for the simultaneous determination of underivatised taurine and methionine in beverages rich in
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