OGS589
PBR322 - PBR322 LOW COPY CLONING VECTOR
plasmid vector for molecular cloning
Synonym(s):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
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About This Item
NACRES:
NA.85
UNSPSC Code:
12352200
Origin of replication:
BR322 (15 copies)
Bacteria selection:
ampicillin
Reporter gene:
none
Peptide cleavage:
no cleavage
Pricing and availability is not currently available.
form
buffered aqueous solution
mol wt
size 4361 bp
bacteria selection
ampicillin
origin of replication
BR322 (15 copies)
peptide cleavage
no cleavage
reporter gene
none
shipped in
ambient
storage temp.
−20°C
General description
This plasmid contains the BR322 (low copy number) origin of replication and provides approximately 20 plasmid copies per bacterial cell. It also contains the mammalian CMV promoter upstream of the MCS and Kan resistance for selection of transfected mammalian cells. Low copy number plasmids are useful for very large transgenes or for transgenes that can be toxic to bacterial cells.
Promoter Expression Level:
Promoter Expression Level:
Application
BR322 Information: BR322 is a wild type plasmid isolated from E.coli. It contains a low copy origin that allows for plasmid maintenance at a frequency of approximately 10-100 plasmid copies per cell. Typically this number is closer to 30-40 copies per cell. Most common cloning plasmids contain a derivative of the origin of replication in this plasmid called pUC. The pUC origin was created by removing the Rep repressor protein that normally regulates plasmid copy number in E.coli and also making a single point mutation in the origin of replication itself. The point mutation is the most important difference between the origin of replication.This plasmid can be used as a general purpose low copy cloning vector.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.oxgene.com
Other Notes
To view sequence information for this product, please visit the product page
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Sigma-Aldrich presents a technical article to help you choose the right SnapFast™ vector to meet your needs.
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual
Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we