Really good value for money and always arrives with a good shelf-life in terms of expiry date. Great for adding cytokines + hydrocortisone to to create your own endothelial cell growth medium - has kept my HUVECs consistently happy since I started using it in 2018. Also great to use when isolating human primary endothelial cells.
M4530
Medium 199
With Earle′s salts, ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Medium 199
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
M199 Medium, TCM 199
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Pricing and availability is not currently available.
Product Name
Medium 199, With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
NaHCO3: yes
L-glutamine: yes
HEPES: no
Earle’s salts (5% CO2): yes
phenol red: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Complete liquid version of the original M 199 formulation published by Morgan.
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Application
Medium 199 has been used:
- as a suspension media to maintain the primary neuronal cultures
- as a component of the collagen gel for ex vivo embryonic heart culture
- to maintain the ovarian tissue sections as part of the slow-freezing protocol
1 of 1
This Item | |||
|---|---|---|---|
| form liquid | form liquid | form powder | form powder |
| sterility sterile-filtered | sterility sterile-filtered | sterility - | sterility - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| components NaHCO3: yes | components sodium pyruvate: no | components L-glutamine: yes | components L-glutamine: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
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Discover the formulation for Medium 199 for cell culture applications, including vaccine development.
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In this report we propose a model in which after the herpes simplex virus (HSV) capsid docks at the nuclear pore, the tegument protein attached to the capsid must be cleaved by a serine or a cysteine protease in order