71077

X-Gal Solution - Novagen

Convenience for blue/white screening

• Synonym(s): X-Gal solution
• UNSPSC Code: 12352200
• NACRES: NA.54

Properties

• Quality Level: 100
• form: solution
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• technique(s): tissue culture: suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

X-Gal, or 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside, is an organic compound commonly used in molecular biology as a chromogenic substrate for the enzyme β-galactosidase, which is encoded by the LacZ gene. When β-galactosidase hydrolyzes X-Gal, it forms galactose and 5,5′-dibromo-4,4′-dichloro-indigo, resulting in the formation of intense blue color colonies. This process is widely used in LacZ activity assays and staining techniques, such as X-gal staining or LacZ staining to distinguish between recombinants (white) and nonrecombinants (blue). X-Gal staining is frequently used in the fields of molecular biology, genetics, and developmental biology to study gene expression and cell line tracing.[1] X-Gal Solution is provided as a convenient 40 mg/ml concentrate in DMSO, ready for dilution into the culture medium or appropriate buffers.

Application

X-Gal Solution - Novagen has been used at 1:40 dilution to detect the expression of β-galactosidase and mutant fusion protein generated by the Apc^Δ242 mutation in the tissue sample.[2]

Warning

Toxicity: Irritant (B)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C