Skip to Content
Merck
All Photos(3)

Documents

B2904

Sigma-Aldrich

Bluo-Gal

reagent for selection of recombinant bacterial clones

Synonym(s):

5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

Empirical Formula (Hill Notation):
C14H16BrNO6
CAS Number:
97753-82-7
Molecular Weight:
374.18
Beilstein:
1550140
MDL number:
MFCD00063691
UNSPSC Code:
12352200
PubChem Substance ID:
24891417
NACRES:
NA.85

grade

for molecular biology

sterility

non-sterile

Assay

≥98% (HPLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for substrate for β-galactosidase

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1

InChI key

LINMATFDVHBYOS-MBJXGIAVSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Principle

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 1

1 of 1

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside ≥98%, powder

Sigma-Aldrich

B4252

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

Leslie M Stone et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(11), 4522-4529 (2002-06-01)
Taste buds are a heterogeneous population of cells exhibiting diverse morphological and biochemical characteristics. Because taste buds arise from multiple progenitors, the different types of taste cells may represent distinct lineages. The present study was undertaken to determine the following:
Ammonium transporter C of Dictyostelium discoideum is required for correct prestalk gene expression and for regulating the choice between slug migration and culmination
Kirsten JH, et al.
Developmental Biology, 287(1), 146-156 (2005)
Delineation of matriptase protein expression by enzymatic gene trapping suggests diverging roles in barrier function, hair formation, and squamous cell carcinogenesis
List K, et al.
The American Journal of Pathology, 168(5), 1513-1525 (2006)
H T Yuan et al.
Journal of the American Society of Nephrology : JASN, 11(6), 1055-1066 (2000-05-23)
Angiopoietin-1 (Ang-1) stimulates endothelial and vascular network differentiation through the Tie-2 receptor tyrosine kinase, while Ang-2 modulates this activation in embryo and tumor growth. The nephrogenic pattern of Ang-2 was documented in a mouse strain that expresses the LacZ reporter
Kyoji Horie et al.
Molecular and cellular biology, 23(24), 9189-9207 (2003-12-03)
The use of mutant mice plays a pivotal role in determining the function of genes, and the recently reported germ line transposition of the Sleeping Beauty (SB) transposon would provide a novel system to facilitate this approach. In this study
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service