05-474
Anti-GABA A Receptor β2/3 Antibody
clone 62-3G1, Upstate®, from mouse
Synonym(s):
GABA(A) receptor subunit alpha-1, gamma-aminobutyric acid (GABA) A receptor, alpha 1, gamma-aminobutyric acid A receptor, alpha 1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
62-3G1, monoclonal
species reactivity
rat, bovine, human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GABRA1(2554)
General description
The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 3 different isoforms of the beta subunit, and receptor subunit expression varies during development. Additionally, GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 and beta 2/3 subunits increase upon chronic exposure to alcohol, and may be potential therapeutic targets for alcohol dependency. The beta 2 subunit is required for receptor assembly and surface expression, while mice with an inactivated beta3 subunit exhibit epilepsy and cleft palate.
Specificity
Specific for the GABAA Receptor β2 subunit, MW ~55 kDa and β3 subunit, MW ~57 kDa.
Immunogen
Affinity-purified GABAA receptor isolated from bovine brain. Clone 62-3G1
Application
Anti-GABA A Receptor β2/3 Antibody, is an antibody against GABA A Receptor β2/3 for use in WB, IP, IH.
Immunoprecipitation: 4 μg of a previous lot immunoprecipitated the GABAA Receptor β2 and β3 subunits from 500 μg of rat brain microsomal tissue extract. Due to interference of the IgG heavy chain, the immunoprecipitated GABAA receptor complex was confirmed using a polyclonal anti-GABAA receptor α1 subunit (Catalog # 06-868).
Immunohistochemistry: This antibody has been reported to detect the GABAA Receptor β2 and β3 subunits in paraffin embedded and frozen rat brain sections which had been fixed with 4% paraformaldehyde or 0.1% glutaraldehyde and permeabilized with Triton X-100.
Immunohistochemistry: This antibody has been reported to detect the GABAA Receptor β2 and β3 subunits in paraffin embedded and frozen rat brain sections which had been fixed with 4% paraformaldehyde or 0.1% glutaraldehyde and permeabilized with Triton X-100.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Quality
Evaluated by western blot on rat brain microsomal preparation.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected the GABAA Receptor β2 and β3 subunits from 20 μg of rat brain microsomal preparation (Catalog # 12-144).
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected the GABAA Receptor β2 and β3 subunits from 20 μg of rat brain microsomal preparation (Catalog # 12-144).
Target description
Varies
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2°C to 8°C from date of receipt.
Analysis Note
Control
Brain tissue
Brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Stoichiometry and assembly of a recombinant GABAA receptor subtype.
Tretter, V, et al.
The Journal of Neuroscience, 17, 2728-2737 (1997)
Marco I González et al.
Epilepsia, 54(4), 616-624 (2013-01-09)
Epileptogenesis is the process by which a brain becomes hyperexcitable and capable of generating recurrent spontaneous seizures. In humans, it has been hypothesized that following a brain insult there are a number of molecular and cellular changes that underlie the
S P Janssen et al.
Neurochemistry international, 60(1), 21-30 (2011-11-24)
Elevated spinal extracellular γ-aminobutyric acid (GABA) levels have been described during spinal cord stimulation (SCS)-induced analgesia in experimental chronic peripheral neuropathy. Interestingly, these increased GABA levels strongly exceeded the time frame of SCS-induced analgesia. In line with the former, pharmacologically-enhanced
Analgesia and hyperalgesia from GABA-mediated modulation of the cerebral cortex.
Luc Jasmin, Samuel D Rabkin, Alberto Granato, Abdennacer Boudah, Peter T Ohara
Nature null
Xuelian Ma et al.
Frontiers in immunology, 9, 987-987 (2018-06-06)
Emerging evidence indicates that gamma-aminobutyric acid (GABA) has many beneficial effects such as ameliorating immune and inflammatory response. But, here we reported that activation of GABAA receptors (GABAA Rs) aggravated dextran sulfate sodium (DSS)-induced colitis, although the expression of pro-inflammatory
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