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RTG-2

90102529, fish ovary, Fibroblast

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About This Item

UNSPSC Code:
41106514

product name

RTG-2, 90102529

biological source

fish ovary

growth mode

Adherent

karyotype

2n = 60

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Trout Rainbow gonad tissue

Cell Line Description

From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24°C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).

Application

Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Passage using 0.25% trypsin below 24°C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO2; 24°C. Fish cell lines detach easily during transit if the culture is very youn

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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