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BF-2

21712, fish caudal trunk, Fibroblast-like

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About This Item

UNSPSC Code:
41106514

product name

BF-2, 00021712

biological source

fish caudal trunk

growth mode

Adherent

karyotype

2n=48, stem-line = 48 with scattering from 36-121

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Fish, bluegill fry

Cell Line Description

The cell line BF-2 has been derived from a trypsinised suspension of pooled caudal portions of the trunk of 1 year old fingerlings of Bluegill fry (Lepomis macrochirus). Cells have been reported to be susceptible to a variety of fish viruses including lymphocystis virus, viral haemorrhagic septicaemia virus (VHSV), infectious pancreatic necrosis virus (IPNV) and tadpole edema virus (TEV) of frogs. The cell monolayer is confluent after 48-56 hours using a 1:4 split ratio. Catalogue no. at CCLV (depositor) is RIE 193.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; cells replicate at 15-33ºC, optimal temperature 23-26ºC; monolayer is stable for at least 10 days, maintenance medium should contain MEM(EBSS) + 740 mg/l NaHCO3 + 1.2g/l

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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