SRP3186

Human Vitronectin

recombinant, expressed in HEK 293 cells, powder, suitable for cell culture

• Synonym(s): Epiboin, S-Protein, Serum-spreading factor, V75, VTN
• CAS Number: 83380-82-9
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• product name: Vitronectin human, recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture
• biological source: human
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• Assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• potency: ≤0.5 μg/mL EC₅₀
• mol wt: 52.2 kDa (calculated)
• packaging: pkg of 250 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white
• solubility: water: soluble
• NCBI accession no.: NM_000638.4
• UniProt accession no.: P04004
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... VTN(7448)

Description

General description

Vitronectin is a secreted glycoprotein which is synthesized in the liver. It is also secreted by respiratory epithelial cells and is present in healthy lungs. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers.The vitronectin gene consists of approximately 4.5–5 kb base pairs with eight exons and seven introns.
Recombinant human Vitronectin is a protein consisting of 459 amino acids. It exists in two forms: a single chain and a clipped form consisting of two chains held together by disulfide bonds resulting from an endogenous cleavage. Recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media.

Application

Vitronectin human has been used to study the effects of ECM (extracellular matrix) ligands on C6 astroglioma cells. It has also been used as an attachment-promoting protein for coating the cell plates.

Biochem/physiol Actions

The multimeric vitronectin protein can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Its interaction with integrin α_vβ₃ enhances hypoxia resistance and tumor cell invasion. It is also associated with cancer progression in various cancers. It is also involved in wound repair and controls complement system as wel. Moreover, vitronectin functions as a marker of profibrotic activity in liver, heart and kidney.

Features and Benefits

• Human-derived vitronectin
• Produced through recombinant expression in HEK 293 cells
• Low endotoxin levels

Sequence

DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL

Physical form

Lyophilized from Phosphate Buffered Saline (PBS), pH 7.4, plus 2.5% trehalose.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

Under reducing conditions, the single chain migrates at an apparent molecular mass of 75kDa on SDS-PAGE, and the clipped form migrates at molecular masses of 65kDa and 10kDa.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable