F2006

Human Fibronectin

from human plasma, powder, suitable for cell culture

• Synonym(s): Fibronectin
• EC Number: 289-149-2
• MDL number: MFCD00131062
• UNSPSC Code: 12352202
• eCl@ss: 42020141
• NACRES: NA.75

Properties

• product name: Fibronectin human plasma, lyophilized powder, BioReagent, suitable for cell culture
• biological source: human plasma
• Quality Level: 200
• sterility: sterile
• product line: BioReagent
• Assay: ≥85% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 450 kDa
• packaging: pkg of 5 x 5 mg; pkg of 1 mg; pkg of 2 mg; pkg of 5 mg
• concentration: >1 mg/mL protein
• technique(s): cell culture | mammalian: suitable
• surface coverage: 1‑5 μg/cm²
• impurities: HBSAG, none detected; HCV, none detected; HIV-1/HIV-2, none detected
• solubility: H₂O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)
• NCBI accession no.: NM_212482.4
• UniProt accession no.: P02751
• Binding Specificity: Peptide Source: Collagen
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... FN1(2335)

Description

General description

Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. The fibronectin structure consists of blocks of repeating peptide sequences that are homologous in nature and form functional unit arranged linearly on two subunit arms. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of fibronectin protein due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

Application

Fibronectin from human plasma is used for the following applications:

• Used in cell seeding and neuronal differentiation (as one of the components in expansion medium for the re-suspension of CBSCs (Cord blood stem cells) and is also used in differentiation medium)
• Used in cell culture (the culture chamber was coated with fibronectin for the expression of HEK293 cells)
• In-vitro fibronectin (FN) pulldown assays
• Protein specificity
• Used in cell migration study

Biochem/physiol Actions

Fibronectin (FN) plays an essential role in multiple biological functions such as, angiogenesis, cell migration cell-to-cell adhesion, cell-to-basement-membrane attachment, clot stabilization, embryogenesis, differentiation, wound healing, as well as binding of pathogen to mammalian cells and extracellular matrix. Studies in Chinese Han population show that mutations in this gene might be linked with susceptibility to knee osteoarthritis (OA). Splice variant extra domain-B (EDB) of FN is a hallmark of tumor angiogenesis, and with increased matrix stiffness there is an increase in the production of this isoform by endothelial cells. In colorectal cancer (CRC), EDA is linked with metastasis, vasculogenesis and tumorigenesis and promotes epithelial-mesenchymal transition.

Caution

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B, hepatitis C and syphilis. In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves