S5171
Human Fibronectin
formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture
Synonym(s):
sFN
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About This Item
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product name
Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in E. coli
product line
BioReagent
form
solution
mol wt
~235-270 kDa
packaging
pkg of 5 mg
technique(s)
cell culture | mammalian: suitable
surface coverage
1 μg/cm2
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... FN1(2335)
Application
Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.
Components
Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.
Caution
Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.
Preparation Note
As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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