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Key Documents

G8673

Sigma-Aldrich

β-Glucanase 2, thermostable

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

β-Glucanase, thermostable

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

liquid

specific activity

≥1.0 units/mg protein

mol wt

38 kDa

concentration

≥20 mg protein/mL (Bradford)

shipped in

wet ice

storage temp.

2-8°C

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Application

β-Glucanase is used to study cell wall modifications and for carbohydrate hydrolysis. It has been used to supplement barley-based diets for poultry and to study the reopening signal conduits and release of dormancy in the Populus species.

Biochem/physiol Actions

β-Glucanase is involved in cell wall modifications and carbohydrate hydrolysis.

Unit Definition

One unit will produce 1 μmole of reducing sugar (measured as glucose) from Beta-glucan per minute at pH 5.8 at 70 °C.

Physical form

Supplied as a solution in 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, and 25% glycerol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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T Ribeiro et al.
Poultry science, 90(6), 1245-1256 (2011-05-21)
To improve the nutritive value of barley-based diet for broilers, 2 experiments using 2 different barley lots were performed to evaluate the capacity of a mesophilic cellulase when fused to a β-glucan specific family 11 carbohydrate-binding module. The data revealed
Päivi L H Rinne et al.
The Plant cell, 23(1), 130-146 (2011-02-02)
In trees, production of intercellular signals and accessibility of signal conduits jointly govern dormancy cycling at the shoot apex. We identified 10 putative cell wall 1,3-β-glucanase genes (glucan hydrolase family 17 [GH17]) in Populus that could turn over 1,3-β-glucan (callose)
Encarnación Dueñas-Santero et al.
Eukaryotic cell, 9(11), 1650-1660 (2010-09-21)
In yeast, enzymes with β-glucanase activity are thought to be necessary in morphogenetic events that require controlled hydrolysis of the cell wall. Comparison of the sequence of the Saccharomyces cerevisiae exo-β(1,3)-glucanase Exg1 with the Schizosaccharomyces pombe genome allowed the identification
Philip A Lessard et al.
Heliyon, 7(8), e07696-e07696 (2021-08-18)
Carbohydrases are often incorporated into livestock feed as digestive aids to improve animal performance. AC1 is a thermostable carbohydrase with β-1,4-glucanase, endo-cellulase, and cellobiohydrolase activity. AC1 has been expressed in corn, where it accumulates in the grain for easy inclusion

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