R1153

RNase B Glycoprotein Standard from bovine pancreas

≥90% (SDS-PAGE), lyophilized powder

• Sinónimos: Ribonuclease B from bovine pancreas, RNase B
• Número de CAS: 9001-99-4
• Número CE: 232-646-6
• Número MDL: MFCD00132184
• Código UNSPSC: 12352204
• NACRES: NA.32

Propiedades

• Nombre del producto: RNase B Glycoprotein Standard from bovine pancreas, Proteomics Grade
• grado: Proteomics Grade
• Nivel de calidad: 200
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: lyophilized powder
• temp. de almacenamiento: −20°C
• cadena SMILES: [nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
• InChI: 1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
• Clave InChI: CQOVPNPJLQNMDC-UHFFFAOYSA-N

Descripción

Aplicación

RNase B is a preferred substrate with PNGase F for demonstration of N-linked deglycosylation using SDS-PAGE or MALDI-MS. The activity of PNGase F is routinely assayed using RNase B by monitoring the pronounced mobility shift in 12% gels after deglycosylation. Proteomics Grade RNase B has been used as a source of N-glycans following enzymatic digestions and subsequent purification. It has also been used as a glycoprotein standard.

Otras notas

Bovine pancreatic RNase B is a glycoprotein that contains only N-linked glycans. It is a globular protein composed of a single domain that occurs naturally as a lesser component in mixture along with ribonuclease A (RNase A), which is the non-glycosylated core form. RNase B contains a single glycosylation site at Asn³⁴ at which from five to nine mannose residues are attached to the chitobiose core, i.e. Man_5-9GlcNAc₂. Due to the heterogeneity in the glycosylation at Asn³⁴, RNase B exists as five glycosylated varients, with a molecular weight of approx. 15 kDa.

Calidad

RNAse B Glycoprotein Standard has been highly purified to remove contaminating RNase A.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)