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Merck

P6998

Sigma-Aldrich

Protein Kinase A Catalytic Subunit β, Active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

Sinónimos:

PKA Catalytic Subunit β, Active human, PKA-Cβ), cAMP-dependent protein kinase

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About This Item

Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.77

recombinante

expressed in baculovirus infected Sf9 cells

Nivel de calidad

Ensayo

≥80% (SDS-PAGE)

Formulario

buffered aqueous glycerol solution

actividad específica

≥10 nmol/min·mg

mol peso

protein apparent mol wt ~65 kDa (including N-terminal GST-tag of ~26kDa)

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Información sobre el gen

human ... PRKACB(5567)

Descripción general

Protein kinase cAMP-activated catalytic subunit β (PRKACB) is one of the catalytic subunits of the serine/threonine kinase, protein kinase A. The gene encoding it is localized on human chromosome 1p36.1.

The molecular weight of about 65kDa is confirmed by the SDS-PAGE gel. The product was generated by expressing recombinant full-length human PKAcβ2 with an additional N-terminal GST tag by baculovirus in Sf9 insect cells. The gene accession number is NM_002731. The resulting protein is 351aa cAMP-dependent protein kinase catalytic subunit beta isoform 2 (NP_002722), or according to UniProt, Isoform 1 (P22694-1).

Aplicación

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.

Acciones bioquímicas o fisiológicas

A catalytic subunit of cAMP-dependent protein kinase, the protein encoded by PRKACB catalyzes events downstream of GPCRs including cell cycle, differentiation and proliferation. When activated, this subunit acts on metabolic enzymes, ion channels and transcription factors such as CREB.
cAMP-dependent protein kinase (PKA; EC 2.7.1.37) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription.

Definición de unidad

One unit will incorporate one nanomole of phosphate into the Crebtide (KRREILSRRPSYR) substrate per minute at 30 deg C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

Forma física

Supplied as a solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Visite la Librería de documentos

Association between PKA gene polymorphism and NTDs in high risk Chinese population in Shanxi.
Wu J, et.al
International Journal of Clinical and Experimental Pathology, 6(12), 2968-2974 (2013)
Jian Wu et al.
International journal of clinical and experimental pathology, 6(12), 2968-2974 (2013-12-03)
This study aimed to investigate the single nucleotide polymorphisms (SNPs) of PKA and neural tube defects (NTDs) in Chinese population. A total of 183 NTDs cases and 200 healthy controls were used in this study. 7 selected single nucleotide polymorphism

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