Saltar al contenido
Merck
Todas las fotos(1)

Documentos

539481-M

Sigma-Aldrich

PKA, Catalytic Subunit, Mouse, Recombinant, E. coli

Sinónimos:

cAMP-Dependent Protein Kinase, Catalytic Subunit, Protein Kinase A, Catalytic Subunit

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Comisión internacional de enzimas:
UNSPSC Code:
12352202

form

liquid

Quality Level

specific activity

≥2.5 x 106 U/mL

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

foreign activity

Phosphatases, proteases, none detected

storage temp.

−20°C

General description

Recombinant, mouse PKA catalytic subunit expressed in E. coli. Regulates a number of cellular functions including gene expression, carbohydrate metabolism, Ca2+ homeostasis, and ion channel function. Suitable for studying the phosphorylation of purified substrates and for use with cell extracts. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation.
Recombinant, mouse PKA catalytic subunit expressed in E. coli. The catalytic subunit of protein kinase A that combines with the regulatory subunit, in the absence of cAMP, to form the inactive PKA holoenzyme. Unlike the holoenzyme, the catalytic subunit does not require cAMP for activation. PKA-Cα is reported to potentiate IP3-induced Ca2+ mobilization in isolated hepatocytes. Suitable for studies of PKA phosphorylation in vitro with purified substrates or following the introduction into intact cells or cell extracts.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will transfer 1.0 pmol phosphate to Kemptide (LRRASLG; Cat. No. 05-23-4900) per min at 30°C, pH 7.5.

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, 2 mM DTT, 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Grant, B.D., and Adams, J.A. 1996. Biochemistry35, 2022.
Lee, S-L. and Steinberg, R.A. 1996. J. Biol. Chem.271, 16553.
Adams, J.A., et al. 1995. Biochemistry 34, 2447.
Bosma, M.M., et al. 1993. J. Neurosci.13, 5242.
Brindle, P., et al. 1993. Nature364, 821.
Hajnoczky, G., et al. 1993. Biochem. J.293, 413.
Sculptoreanu, A., et al. 1993. Proc. Natl. Acad. Sci. USA90, 10135.
Wang, L-Y., et al. 1993. Science259, 1173.
Slice, L.W., and Taylor, S.S. 1989. J. Biol. Chem.264, 20940.
Uhler, M.D., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1300.
Walsh, D.A., et al. 1968. J. Biol. Chem.243, 3763.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico