Product P1944 - Phenol:Chloroform: Iso-amyl alcohol (125:24:1) is acidic. As such, the RNA isolates in an acidic pH and will partition the genomic DNA into the organic phase leaving only the RNA in the top aqueous phase.
P1944
Phenol:Chloroform:Iso-amyl alcohol (125:24:1)
for molecular biology
Sinónimos:
phenol chloroform RNA extraction
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About This Item
Código UNSPSC:
12190000
NACRES:
NA.52
grado:
for molecular biology
Ensayo:
≥99.0%
aplicaciones:
life science and biopharma
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
grado
for molecular biology
Nivel de calidad
Ensayo
≥99.0%
Formulario
liquid
pH
4.3-4.7
aplicaciones
life science and biopharma
temp. de almacenamiento
2-8°C
Descripción general
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.[1]
Aplicación
Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2
Órganos de actuación
Liver,Kidney, Nervous system,Kidney,Liver,Skin
Código de clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
175.0 °F - closed cup
Punto de inflamabilidad (°C)
79.44 °C - closed cup
Equipo de protección personal
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
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Hello, Your product is fractionated into two phases. Should I use lower phase for RNA extraction? Thank you
1 answer-
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