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Merck

MAK083

Sigma-Aldrich

Glucose Uptake Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84
En este momento no podemos mostrarle ni los precios ni la disponibilidad

uso

sufficient for 100 colorimetric tests

método de detección

colorimetric

enfermedades relevantes

endocrinological disorders, diabetes; cancer

temp. de almacenamiento

−20°C

Descripción general

Glucose is the primary source of energy for most cells. Glucose uptake into cells is highly regulated and the first rate limiting step in glucose metabolism. Glucose uptake is facilitated by the GLUT family of transporter proteins, whose expression and activity are regulated by multiple mechanisms. Glucose uptake is upregulated in many cancer cells, which exhibit high rates of aerobic glycolysis. Cells exhibiting insulin resistance show diminished glucose uptake in response to insulin stimulation.

The use of the recycling amplification reaction in the colorimetric assay results in the limit of detection being 10-fold lower (20-100 pmole) compared to the fluorescence assay (200-1,000 pmole, MAK084).

Aplicación

Glucose uptake colorimetric assay kit has been used to measure glucose uptake by a variety of cells.[1][2][3]

Idoneidad

Suitable for detecting glucose uptake in adherent or suspension cells cultured in a 96-well microtiter plate.

Principio

The Glucose Uptake Colorimetric Assay kit provides a simple and direct procedure for measuring glucose uptake in a variety of cells. Glucose uptake is measured using the glucose analog, 2-deoxyglucose (2-DG), which is taken up by cells and phosphorylated by hexokinase to 2-DG6P. 2-DG6P cannot be further metabolized and accumulates in cells, directly proportional to the glucose uptake by cells. In this assay, 2-DG uptake is determined by a coupled enzymatic assay in which the 2-DG6P is oxidized, resulting in the generation of NADPH, which is then determined by a recycling amplification reaction in which the NADPH is utilized by glutathione reductase in a coupled enzymatic reaction that produces glutathione. Glutathione reacts with DTNB to product TNB, which is detected at 412 nm.

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Environment

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Clasificaciones de peligro

Aquatic Chronic 2

Código de clase de almacenamiento

10 - Combustible liquids


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Obesity is characterized by an excessive accumulation of fat in adipose tissue, which is associated with oxidative stress and chronic inflammation. Excessive H2O2 levels are degraded by catalase (CAT), the activity of which is decreased in obesity. We investigated the

Questions

  1. What color should be perceived when the reaction mix B is added to the samples/standards?

    1 answer
    1. The expected color is yellow to yellowish orange. The color of the standard may be easier to perceive as the standard is pure 2-DG6P, compared to a sample, where it is concentration dependent.

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