This product has not been validated for deparaffinization of formalin-fixed, paraffin-embedded samples. However, an article in "Applied Immunohistochemistry & Molecular Morphology" (2014 Sep;22(8):623-6) indicated that mineral oil appears to work well for deparaffinizing these samples and for DNA extraction.
M8410
Aceite de inmersión
light oil, (neat)
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About This Item
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
esterilidad
non-sterile
Formulario
light oil
condiciones de almacenamiento
protect from light
índice de refracción
n20/D 1.467 (lit.)
densidad
0.84 g/mL at 25 °C (lit.)
Formato
neat
Clave InChI
AEOVEGJBKQQFOP-DDVLFWKVSA-L
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Aplicación
Precaución
Este producto no es estéril. El aceite mineral es difícil de esterilizar. Sólo puede ser esterilizado mediante filtración cuidadosa del aceite calentado. Se supone que se añadirán antibióticos al medio de cultivo. De lo contrario deberá utilizarse el producto de referencia M5310, que está esterilizado por filtración.
Nota de análisis
Este material se ha analizado como recubrimiento en cultivos embrionarios en microgotas. (Todos los materiales analizados se prueban utilizando embriones de ratón.) Se utilizan normalmente los híbridos BCF1 x BDF1 El aceite se utiliza fundamentalmente para evitar la evaporación del medio, en particular cuando se realizan manipulaciones en el embrión.1 El procedimiento es el siguiente: Embriones de una célula (BCF1 x BDF1) se cultivaron en microgotas por triplicado del medio probado en embriones HTF (líquido tubárico humano) complementado con BSA al 0,4%. Para un análisis válido, al menos el 70% de los embriones de control en estadio de una célula deben desarrollarse al estadio de blastocisto en 96 horas. Para la liberación del producto, al menos el 80% de los embriones problema en estadio de una célula deben desarrollarse al estadio de blastocisto en 96 horas con el material analizado.
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
No data available
Punto de inflamabilidad (°C)
No data available
Equipo de protección personal
Eyeshields, Gloves
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Artículos
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Can M8410 Sigma-Aldrich Mineral oil be used for the deparaffinization of FFPE samples, and is it compatible with downstream histological or molecular analyses like immunohistochemistry or RNA/DNA extraction?
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Hello,I´m looking for a mineral oil for sulfur pattern production. It´s difficult to find an oil in which the content of sulfur compounds appears in the COA. Can you inform if there´s a possibility of sulfur contamination in the production of mineral oil?
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The manufacturing process for this product is considered proprietary. Currently, there is no product option available that is tested for the presence of sulfur.
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ıs this suitable for preparation of carbon paste electrode?
1 answer-
This material has not been validated in the process of making carbon paste electrodes. However, if the specifications (found below) meet your requirements, then there is no reason that they can't be used in this process.
https://www.sigmaaldrich.com/specification-sheets/990/164/M8410-BULK.pdfHelpful?
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Hello, Does this product have an odour? I want to use it for an olfactory memory assay, so needs to be odourless
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According to Section 9 of the Safety Data Sheet, this is an odorless chemical.
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What is the composition of the oil? Where does it comes from?
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M8410 is a light oil. Mineral oil is a complex mixture of different hydrocarbons and its typical composition is as follows:
Paraffinic oils, based on n-alkanes, 15-60%
Naphthenic oils, based on cycloalkanes, 30%-60%
Aromatic oils, based on aromatic hydrocarbons, 3-30%
Light and heavy mineral oil differ in terms of composition, viscosity, and density.
Mineral oil with an alkyl chain carbon number of 15 to 25 is categorized as light oil whereas 25 to 50 as heavy oil.
Light oil has a density of 0.83–0.86 kg L-1 whereas heavy oil has 0.875–0.905 kg L-1
Light mineral oil is less occlusive than heavy mineral oil as it contains more cyclic (saturated) moleculesAdditional Links: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/326/294/m8410pis.pdf and a second document: https://www.sigmaaldrich.com/specification-sheets/990/164/M8410-BULK.pdf
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