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L3892

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

peroxidase conjugate, lyophilized powder

Sinónimos:

WGA, Wheat germ agglutinin

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

origen biológico

Triticum vulgaris

Nivel de calidad

200

conjugado

peroxidase conjugate

Ensayo

≥85% protein basis (Warburg-Christian)

Formulario

lyophilized powder

potencia

<40 μg/mL agglutination activity

peroxidase activity

50-200 units/mg protein

composición

Protein, ~90% modified Warburg-Christian

temp. de almacenamiento

−20°C

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Aplicación

Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Acciones bioquímicas o fisiológicas

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Envase

Package size based on protein content.

Definición de unidad

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Forma física

Contains citrate buffer

Nota de preparación

Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000379556
0000304765
0000304766
0000304765
0000379556

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