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Key Documents

I5287

Sigma-Aldrich

Human IgG2 Lambda,Recombinant Protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

human

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

assay

>95% (SDS-PAGE)

shipped in

dry ice

storage temp.

−20°C

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
IgG2, λ recombinant protein is obtained by expression of a phage library in a human cell line and purified by affinity chromatography.

Application

The purified IgG2, λ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4

Analysis Note

The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg


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