Yes, the difference between product H3667 and H4522, is that product H3667 is heat inactivated. Any availability questions can be directed to customer service. Please navigate to the link below:https://www.sigmaaldrich.com/support/customer-support.
H3667
Suero humano
Human Serum
Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered
Sinónimos:
plasma humano, suero humano, sueros humanos
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About This Item
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
human male plasma (Type AB)
Nivel de calidad
esterilidad
sterile-filtered
composición
hemoglobin, ≤20 mg/dL
origen
USA origin
técnicas
cell culture | mammalian: suitable
impurezas
≤10 EU/mL endotoxin
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
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Descripción general
El suero se requiere principalmente como suplemento para los medios de cultivo celular. Contiene macromoléculas, proteínas transportadoras, factores de fijación y de propagación, nutrientes de bajo peso molecular y hormonas y factores de crecimiento.[1]
Aplicación
Envase
El embalaje puede variar para diferentes lotes debido a la escasez de las botellas utilizadas para embalar este producto. La presencia o falta de envoltura retráctil alrededor de la tapa de la botella no afecta a la integridad/esterilidad del producto.
Nota de análisis
Analizada la presencia de endotoxinas
Otras notas
No es inusual que nuestros productos séricos humanos estén turbios. Este producto procede de la combinación de sueros y la turbidez indica la presencia de lípidos en el suero. No se dispone de productos séricos humanos transparentes, carentes de lípidos.
Cláusula de descargo de responsabilidad
SÓLO PARA INVESTIGACIÓN. Este producto está regulado en Francia cuando está destinado a ser utilizado con fines científicos, como para actividades de importación y exportación (artículo L 1211-1, apartado 2, del Código de Salud Pública). El comprador (es decir, el usuario final) está obligado a obtener una autorización de importación del Ministerio de Investigación de Francia referida al artículo L1245-5-1 II del Código de Salud Pública. Al pedir este producto, usted confirma que ha obtenido la autorización de importación adecuada.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
Lot/Batch Number
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Protocolos
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Is the only difference between H3667 and H4522 that H3667 is heat inactivated whilst H4522 is not? And what makes a big difference in the availability between them?
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Is this tested for Anti HLA antibodies? I am thinking of it’s application as a flow cytometric negative control
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This product has not been tested for the presence of anti-HLA antibodies.
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Why is serum Heat-Inactivated (HI)?
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Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the difference between human serum from AB plasma and human serum from AB clotted whole blood?
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Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present. The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma
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