The molecular weight of this enzyme has not been determined. However, Biochem. Biophys. Res. Comm. 59, 894 (1974) reports a molecular weight of 55,000 daltons for the monomer. The enzyme is functionally active as a dimer or tetramer.
G5885
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
lyophilized powder, >= 550 units/mg protein (biuret)
Sinónimos:
G-6-P-DH
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About This Item
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Productos recomendados
origen biológico
bacterial (Leuconostoc mesenteroides)
Nivel de calidad
tpo
Type XXIV
Formulario
lyophilized powder
actividad específica
>= 550 units/mg protein (biuret)
mol peso
128 kDa
composición
Protein, 15-40% biuret
aplicaciones
agriculture
actividad extraña
6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%
PGI ≤0.01%
temp. de almacenamiento
2-8°C
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Descripción general
Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer.[1] Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large β + α domain and has a unique aspartate residue at position 374.[2]
Aplicación
Acciones bioquímicas o fisiológicas
Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP+) or NAD+ as coenzyme making it crucial for bacterial metabolism.[2]
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.
Definición de unidad
One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.
Forma física
Lyophilized powder containing Ficoll and Tris buffer salts
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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M S Cosgrove et al.
Biochemistry, 39(49), 15002-15011 (2000-12-07)
The role of Asp-177 in the His-Asp catalytic dyad of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides has been investigated by a structural and functional characterization of the D177N mutant enzyme. Its three-dimensional structure has been determined by X-ray cryocrystallography in
V Vought et al.
Biochemistry, 39(49), 15012-15021 (2000-12-07)
The roles of particular amino acids in substrate and coenzyme binding and catalysis of glucose-6-phosphate dehydrogenase of Leuconostoc mesenteroides have been investigated by site-directed mutagenesis, kinetic analysis, and determination of binding constants. The enzyme from this species has functional dual
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FEBS letters, 291(2), 233-237 (1991-10-21)
The standardized enzyme coupling method for assaying sucrose synthase activities in the direction of sucrose cleavage was reexamined using enzyme preparations from cultured cells of sycamore (Acer pseudoplatanus L.) and spinach leaves (Spinacea oleracea). Both ATP and Tris, commonly utilized
A simple ultramicro method for determination of pyridine nucleotides in tissues.
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Protocolos
To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.
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What is the molecular weight of Product No. G5885, Glucose-6-phosphate Dehydrogenase?
1 answer-
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In which solvent can Product No. G5885, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?
1 answer-
The product can be dissolved at 1 mg/ml in 5 mM glycine buffer , pH 8, (with or without 0.1% BSA)or in deionized water at 1 mg/ml (buffer is preferable).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?
1 answer-
Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli. Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.
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Can solutions of Product No. G5885, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc mesenteroides, be stored?
1 answer-
Solutions in buffer may be aliquotted and stored frozen at -20°C for about 1-2 months.
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