F5512
Anti-Human IgG (Fab specific)−FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Sinónimos:
Goat Anti-Human IgG (Fab specific)−Fluorescein isothiocyanate
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About This Item
Productos recomendados
origen biológico
goat
Nivel de calidad
conjugado
FITC conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
Formulario
buffered aqueous solution
condiciones de almacenamiento
protect from light
técnicas
immunofluorescence: 1:16-1:32 using Hep2 cells
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Descripción general
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
The product binds all human Igs and provides reduced background with mouse or rat samples.
Aplicación
Anti-Human IgG (Fab specific)-FITC antibody has been used:
- in fluorescent staining
- in dual color fluorescent labeling
- in fluorescence-activated cell sorting (FACS) analysis
Anti-Human IgG (Fab specific)-FITC antibody produced in goat may be used at a working antibody dilution of 1:16 - 1:32 for anti-nuclear antibody (A.N.A.) assay on acetone-fixed human Hep2 cells. This antibody was used in FACS of HNE2 and KNE2/LMP1 cells. It was also used to perform TARDIS assay for detection of topoisomerases I and II that are covalently bound to DNA in K562 leukemia cells.
Acciones bioquímicas o fisiológicas
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Human IgG (Fab specific) antibody allows for the visualization of protein by fluorescent microscopy.
Otras notas
No cross-reaction with mouse and rat IgG.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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