E6126
Enolase from baker′s yeast (S. cerevisiae)
lyophilized powder, ≥50 units/mg protein
Sinónimos:
2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase
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About This Item
Productos recomendados
Formulario
lyophilized powder
Nivel de calidad
actividad específica
≥50 units/mg protein
mol peso
93 kDa
composición
Protein, 50-80% modified Warburg-Christian
solubilidad
15 mM Tris HCl, pH 7.4: soluble 1.0 mg/mL, clear
temp. de almacenamiento
−20°C
Descripción general
Enolase (2-phosphoglycerate hydratase) is a cytosolic enzyme. This cytosolic metalloenzyme is encoded by three homologous genes in mammals. Enolase the from baker′s yeast is a homodimer containing two bound Mg2+ ions. The peptide consists of 436 amino acids and contains a single cysteine residue and corresponds to a molecular weight of 93 kDa. Two of the active site components include His191 and Arg414. Enolase is seen in archaea, bacteria and eukaryotes.
Aplicación
Enolase from baker′s yeast (S. cerevisiae) has been used as a supplement in assay buffer for phosphoglycerate mutase (PGM) enzyme assays.
Enolase from baker′s yeast has been used in a study to investigate the contribution of the antibodies response induced by a low virulent Candida albicans strain in protection against systemic candidiasis. Enolase from baker′s yeast has also been used in a study to investigate the role of metal ions in catalysis by enolase.
The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis.
Acciones bioquímicas o fisiológicas
Enolase is a metalloenzyme that catalyzes the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase is essential for both glycolysis and gluconeogenesis.
Enolase plays a key role in glycolysis and gluconeogenesis. Enolase, found in the nucleus of mammalian cells plays a key role in transcriptional modulation of genes, which participates in morphological transformation and cell proliferation.
The phosphorylated tyrosine residue present in yeast enolase forms a substrate for phosphorylation by tyrosine protein kinase. Apart from Mg2+, the enzyme can be activated by Zn2+, Mn2+, and Cd2+.
Definición de unidad
One unit will convert 1.0 μmole of 2-phosphoglycerate to phospho(enol)pyruvate per min at pH 7.4 at 25 °C.
Forma física
Lyophilized powder containing Tris buffer salts
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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J A Cooper et al.
The Journal of biological chemistry, 259(12), 7835-7841 (1984-06-25)
Enolase, lactate dehydrogenase, and phosphoglycerate mutase have previously been found to contain phosphotyrosine in fibroblasts transformed by Rous sarcoma virus, which encodes a tyrosine-specific protein kinase. However, these phosphorylations are not stoichiometric, and their significance for any aspect of the
T Orth et al.
Clinical and experimental immunology, 112(3), 507-515 (1998-07-02)
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Enolase: a key player in the metabolism and a probable virulence factor of trypanosomatid parasites?perspectives for its use as a therapeutic target
AvilAn L, et al.
Enzyme Research, 2011 (2011)
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