D2532

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

• Sinónimos: blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution
• Código UNSPSC: 12161700
• NACRES: NA.25

Propiedades

• Formulario: solution
• técnicas: Southern blotting: suitable
• actividad extraña: DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Componentes

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable