CHO-K1 Cell Line from hamster

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85051005, hamster ovary, Epithelial

• Sinónimos: CHO cell clone K1 Cells, CHOK1 Cells
• Código UNSPSC: 41106514

Propiedades

• origen biológico: hamster ovary
• envase: tube of 5 μg 85051005-DNA-5UG; pkg of vial of cells 85051005-1VL
• modo de crecimiento: Adherent
• cariotipo: Hypodiploid, modal no. 20
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Hamster Chinese ovary

Descripción línea celular

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.

Aplicación

CHO-K1 cell line from hamster has been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.

Nutritional and gene expression studies

Medio de cultivo

Ham′s F12+ 2mM Glutamine+ 10% Foetal Bovine Serum FBS / FCS.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25%Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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