The maximum size of cell clumps that can pass through the Scepter 3.0 is determined by the aperture limits, which are 40um and 60um for sensors 40 and 60, respectively. Unlike the Scepter 2.0, the Scepter 3.0 does not use a screen to break up cell clump aggregates; instead, it employs a set of pillars to achieve this function. In contrast, the Scepter 2.0 utilizes a 35um Nylon filter to break up cell clump aggregates.
PHCC360KIT
Contador celular automático que cabe en la palma de la mano Scepter 3.0
Rapid cell counts in a handheld easy to use format, includes pkg of 60 μm Scepter 3.0 Cell Counter Sensors
Sinónimos:
Cell counting instrument
About This Item
Productos recomendados
fabricante / nombre comercial
Scepter
técnicas
cell counting: suitable
tamaño de partícula
6-36 μm
compatibilidad
for use with cells sized 4.0–25.0 μm
Condiciones de envío
ambient
Descripción general
- Concentración celular
- Tamaño celular promedio
- Volumen celular promedio
- Histograma de distribución por tamaño o volumen
El contador celular Scepter 3,0 es compatible con los sensores del contador celular Scepter 3.0 de 40 uM y 60 uM. Este kit (PHCC360KIT) contiene un paquete gratuito de 50 sensores de 60 uM.
Componentes
- 1 Contador de células automático que cabe en la palma de la mano Scepter 3.0
- 1 Vial de microesferas problema
- 1 Estación de carga
- 50 Sensores (60μM)
Otras notas
Para utilizar con
Código de clase de almacenamiento
10-13 - German Storage Class 10 to 13
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.
Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.
Data for validated human cell lines using the Millicell® DCI Digital Cell Imager for confluency determination and estimated cell counts.
Protocolos
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Contenido relacionado
Optimice la captura y el análisis de los datos de TEER (resistencia eléctrica transepitelial) con mejoras fáciles de utilizar que abarcan una interfaz de pantalla táctil intuitiva, una sonda independiente para introducir en los pocillos y el registro automático de los datos.
Optimice la captura y el análisis de los datos de TEER (resistencia eléctrica transepitelial) con mejoras fáciles de utilizar que abarcan una interfaz de pantalla táctil intuitiva, una sonda independiente para introducir en los pocillos y el registro automático de los datos.
Optimice la captura y el análisis de los datos de TEER (resistencia eléctrica transepitelial) con mejoras fáciles de utilizar que abarcan una interfaz de pantalla táctil intuitiva, una sonda independiente para introducir en los pocillos y el registro automático de los datos.
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What is the maximum size of cell clumps that can pass through the Scepter 3.0? Also, what size screen is used to break up the cell clump aggregates in both Scepter 2.0 and 3.0?
1 answer-
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Is this appropriate for size 15-23 μm cell? Does the Kit include also the charger? How many 60/40 μm sensors are included Which sensors can be used with this instrument? Which sensors are appropriate for cells size 15-23 μm and what is their price?
1 answer-
Please see the answers to the following questions below:
1. Is this appropriate for size 15-23 μm cells?
Yes, the Scepter 3.0 is suitable. As listed on the product detail page in the DESCRIPTION section for use with cells sized 4.0–25.0 μm.2. Does the Kit include the charger?
This product does include 60μM sensors and a charging station.3. How many 60/40 μm sensors are included
As mentioned on the product detail page in the DESCRIPTION section under 'Components', it includes 50 Sensors.4. Which sensors can be used with this instrument?
This product is only compatible with Scepter 3.0 Cell Counter Sensors shown in the Related Products on this page. Please see the link below to review additional information regarding the Scepter 3.0:
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-counting-and-health-analysis/scepter-cell-counter5. Which sensors are appropriate for cells size 15-23 μm and what is their price?
See question 1, this product is suitable for the cell size of 15-23 μm.Regarding pricing, Please navigate to the link https://www.sigmaaldrich.com/support/customer-support, and click on "Products Pricing & Availability" under the Products Section with all the required information so that a member of the Customer Service team can reach out to assist further. Thank you.
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Does it require Trypan blue or another dye? Does it distinguish between live and dead cells?
1 answer-
The Scepter is a particle counter, but it will not differentiate live from dead cells. The device can be used to gauge cell health on the basis of cell size. For example, in cell types where apoptotic cells are smaller in size compared to non-apoptotic cells. This shift in size can be quantified using the device option "Edit Histogram" to either gate around this population or exclude the population from the count.
Please see the following manual below for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/799/484/00110230w-rev1020-ms.pdfHelpful?
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