C8118

Chymase human

recombinant, expressed in Pichia pastoris

• Sinónimos: Mast cell protease I
• Número CAS: 97501-92-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• Número CE: 3.4.21.39 (BRENDA, IUBMB)
• MDL number: MFCD00132173
• Specific activity: ≥40 units/mg protein
• Recombinant: expressed in Pichia pastoris
• Concentration: 125-400 μg/mL

Propiedades

• recombinant: expressed in Pichia pastoris
• Quality Segment: 300
• form: liquid
• specific activity: ≥40 units/mg protein
• mol wt: ~37 kDa by SDS-PAGE
• concentration: 125-400 μg/mL
• UniProt accession no.: P23946
• application(s): diagnostic assay manufacturing
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... CMA1(1215)

Descripción

General description

Chymase is a cathepsin G-like, S1 serine proteinase found primarily in mast cells. It has a molecular mass of ~30 kDa, however its apparent molecular mass on SDS-PAGE is around 37 kDa.

Application

Chymase has been implicated in generation of angiotensin II and cleavage of big endothelin. Studies indicate it may be involved in vascular proliferation, myocardial infarction and dermatitis.

Human chymase has been used in a study to assess the effects of Panax notoginseng flower extract on the TGF-β/Smad signal transduction pathway in heart remodeling. Human chymase has also been used in a study to investigate the blood glucose level and survival in streptozotocin-treated human chymase transgenic mice.

Biochem/physiol Actions

The enzyme rapidly converts angiotensin I to angiotensin II. Optimum pH for the enzyme activity is between 7.5 and 9.0. Enzyme activity is inhibited by soybean trypsin inhibitor, phenylmethylsulfonyl fluoride and chymostatin.

Physical form

Supplied as a solution in 20 mM Tris, 0.8 M NaCl and 25% glycerol, pH 7.6

Other Notes

One unit hydrolyzes one micromole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7.8 and 25 °C. The assay buffer used to determine the enzyme activity contains 27 mM Tris-HCl, pH 7.8, with 150 mM NaCl and 0.43 mM BTEE.

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)